Method to determine the content of hard metals in whole blood

Professor Nina V. Zaitseva, PhD, DSc, Professor Tatyana S. Ulanova, PhD, DSc, Plakhova L.V., Suetina G.N., Stenno E.V.

FIELD: medicine.

SUBSTANCE: the tested sample is treated with an acid followed by its ashing, the ash obtained is treated with a concentrated mineral acid followed by evaporation up to the state of a moist salt, then a background solution is conducted and the content of metals is determined by the instrumental method. As an acid to treat the sample tested and as a background solution one should use 0.5-5.0% aqueous solution of nitric acid at the sample: acid weight ratio being 1:1. Moreover, before ashing the sample should be dried in two stages at 110 С and 250 C, correspondingly; ashing is соnducted at 430 С. As а соncentrated mineral acid to treat the obtained ash it is necessary to use a concentrated nitric acid and as an instrumental method one should use an atom-absorptive spectrophotometry.

EFFECT: higher accuracy of determination, broadened spectrum of detected components within 1-6 samples at their mutual availability (manganese, lead, nickel, zinc, copper, chromium) and decreased volume of biomaterial necessary for an assay.

Registered RU 2 184 973 C1

G 01 N 38/84

Application: 2001114386/14, 24.05.2001

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