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Method of quantitative determination of manganese, lead and nickel in bile by method of atomic-absorption analysis with atomisation in flame

Year: 
2009
Autors: 
Professor Nina V. Zaitseva, PhD, DSc, Professor Tatyana S. Ulanova, PhD, DSc, Legotkina G.I., Stenno E. V., Bakanina M. A., Shardakova J. V.

FIELD: chemistry.

SUBSTANCE: invention relates to laboratory methods of analysis and deals with method of quantitative determination of manganese, lead and nickel in bile by method of atomic-absorption analysis with atomisation in flame. Essence of method lies in the following: sampling of bile is carried out during duodenal probing, after that it is frozen, and unfrozen at room temperature, homogenisation of bile by mixing being performed already at partial soft unfreezing. After that, sampling of homogenised bile is carried out for preparation for analysis, concentrated nitric acid is introduced into it with volume ratio 1:1, mixture is kept at room temperature, then heated and further mixture is kept for not less than 2.5 hours at room temperature. In order to obtain analyte, to obtained mixture added is concentrated hydrogen peroxide in volume ratio 1:1 to volume of bile sample volume, analyte is heated, after that cooled to room temperature. After that by method of atomic-absorption spectrometry, using graduated diagram, quantitative content of particular type of metal: manganese, lead and nickel is determined in analyte.

EFFECT: invention allows increasing accuracy of quantitative determination of manganese, lead and nickel in bile.

Registered RU 2 410 691 C1

Application: 2009129998/15, 04.08.2009

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